Introduction: Patient safety is one of the important components in quality improvement of health services. This study aimed to evaluate aspects of patient safety culture and identification of intervention priority in the Seidolshohada Hospital. Method: The study was a cross – sectional on the community of nurses, midwives, and the staff in radiology and laboratory. Sampling method was census. Data collection was conducted using standard questionnaire of hospital survey on patient safety culture (HSOPSC). The data was analyzed descriptive statistics (frequency, percentage, mean) using software spss-16. According questionnaire guide in each aspects average at least 70 percent positive safety culture, safety culture medium 70-50, and 50 were considered poor safety culture. Results: In this study, the largest group participant was nurse / midwife (66.7%). Most participants work experience 5-1 years (46.7%) and 59-40 hours worked per week (46.7%). The interaction with the patients, 95% of participants in direct contact with patients and 5% indirect communication with patients. The dimension that received the highest score was "organizational learning and continuous improvement" (74.5%) and the dimension with the lowest scores was "Non-punitive response to error" (49.1%). The other aspects of safety culture was at a moderate level. Conclusion: This study emphasizes the need to promote a culture of patient safety in terms of expectations and the Supervisor/ Manager to improve safety, communication and provide feedback on the errors, the frequency of reporting accidents, improve teamwork between the unit and the non-punitive response to error.