Introduction: Adolescence is a very important stage of any person’s life, since it has a relationship with health. Adolescent females have a special importance among these groups. It seems that having knowledge about health principles in the puberty period leads to the prevention of many misunderstandings and problems in adolescents. Therefore, the effect of puberty health education on awareness about health assessment and general health in females studying in junior high schools of Qain city was assessed.

Methods: This research was an experimental and pre-test design with a control group. The research sample included students in 2 different 3rd year classes of junior high schools. The samples were divided with random sampling to an intervention (N = 47) and control group (N = 40). After the intervention, information was collected with awarness health assessment and general health questionnaires. An education program about puberty and menstrual hygiene for students in the intervation group was offered with lectures, face to face, question and answer, discussions during three 60-minute sessions. The data were analyzed through using t test, chi-square, Wilcoxon, Mann Whitney, Pearson, Analysis of Variance (ANOVA) and repeated measures, using the SPSS 18.

Results: T independed test showed that awarerness health assessment score of students about health the in intervention group, 1 month after education, had a significant difference with the control group (P = 0.039). However, this difference was not significant immediately after education between the 2 groups (P = 0.139). Mann Whitney test showed that general health score students in the intervention groups and 1 month after education showed significant differences with the control group (P = 0.44). The Wilcoxon test showed that there was no significant difference in the 2 groups between the scores of general health before and 1 month after the training.

Conclusions: Although adolescent health education could not significantly increase students' general health score, yet is managed to some extent by increasing awareness health assessment of students, which is effective in improving health score. Therefore, it is essential that the authorities have to pay greater attention to the problems of females in this period.