Introduction: Research has shown that a lot of managerial and organizational factors are involved in burnout. The purpose of this research was to determine the relationship between spiritual leadership, perceived organizational support with job burnout among nurses working at the hospitals of Isfahan Medical University.

Methods: The design of this research was descriptive and correlation. The total number of nurses working at the studied unit was 4000, and 351 individuals were chosen based on Cochran’s sample size formula. The tools used in this research included a spiritual leadership questionnaire of Fry et al. (2005), the organizational support of Eisenberger et al. (1986) and job burnout of Maslach (1981).

Results: The face and content validity of all three questionnaires was confirmed. The reliability of the questionnaires was calculated using Coronbach’s alpha (0.78, 0.83 and 0.74, respectively). The data analysis was performed with the use of step by step regression, Pearson coefficient and variance analysis test.

Conclusions: The results showed that there is a meaningful and reverse relationship between spiritual leadership (r = -0.191, P < 0.01), vision, faith, membership, organizationl commitment, organizational support and job burnout (r = -0.203, P < 0.01). The best prediction is organizational support of job burnout (P < 0.01).